Our lab is interested in studying development, modulation and derangements of synaptic transmission at central nervous system synapses. The human disease processes most relevant to our studies include epilepsy, hypoglycemic brain injury and brain injury following stroke. Our experimental preparations are cultured neurons, acutely prepared live brain slices, and live rodents (transgenic mice, Sprague-Dawley rats), and our methods include single cell, brain slice and in vivo electrophysiology, histology (including immunohistochemistry and confocal imaging), and behavioral studies of live animals. Our previous studies in cell culture examined modulation of AMPA-type glutamate receptor desensitization by thiazides, their effects upon glutamatergic synaptic transmission, and their influence upon excitotoxic neuronal and glial cell death. Our current studies include hypoglycemic brain injury in young rats as a model for hypoglycemia-induced brain dysfunction in Type 1 diabetic children, the neurophysiological functions of fibroblast growth factor 14 (FGF14), and the antiepileptic and anorexigenic effects of ketogenic diets.